Effect of Electroacupunture on Gastric Mucosal Intestinal Trefoil Factor Gene Expression of Stress-induced Gastric Mucosal Injury in Rats.
From: Institute of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China.
World journal of gastroenterology : WJG
- Publish Date: Mar 2006
- ISSN: 1007-9327
- Volume: 12
- Issue: 12
- Pages: 1962-5
- Medium: Print
- Language: English
- Citation (JAMA): Li Xi-Ping, Yan Jie, Yi Shou-Xiang, et al. Effect of Electroacupunture on Gastric Mucosal Intestinal Trefoil Factor Gene Expression of Stress-induced Gastric Mucosal Injury in Rats.. World J. Gastroenterol. Mar 2006;12:1962-5
Abstract
AIM: To investigate electroacupuncture(EA) at the acupoints of Stomach Meridian of Foot-Yangming (SMFY), Gallbladder Meridian of Foot-Yangming (SMFY) on gastric mucosal intestinal trefoil factor (ITF) gene expression detection in stress-induced rats with gastric mucosal lesion, and to explore the regulatory mechanism and significance of EA-related gastric mucosal protective effect. METHODS: Forty rats were randomly divided into 4 groups: Blank group, Model group, Model group+EA at acupoints of SMFY group (“SMFY group”), and Model group+EA at acupoints of GMFY group(GMFY group). All rats (except blank group) were made model by water immersion and restraint stress (WRS). Then the gastric mucosa tissue in each rat was taken off after assessment of gastric mucosal lesion index(GUI), and the expression of ITF mRNA of the tissues was detected by reverse transcription-polymerase chain reaction (RT-PCR) method. RESULTS: Compared with Model group(54.3+/- 1.34), the GUI value in SMFY group (31+/- 2.21) decreased significantly(P< 0.01), so did that in GMFY group (39.8+/- 1.62, P< 0.05), meanwhile GUI value in SMFY group was significantly lower than in GMFY group(P< 0.01). Compared with Model group (0.65+/- 0.01), EA had a tendency to improve the expression of gastric mucosal ITFmRNA gene: such tendency existed in GMFY group (0.66+/- 0.01) but with no significant difference(P>0.05), in SMFY group(0.76+/- 0.01) with an extremely obvious difference (P< 0.01), furthermore the expression in SMFY group was significantly higher than in GMFY group (P< 0.01). CONCLUSION: The gastric mucosal protective effect by EA at the acupoints of SMFY and GMFY was related to the expression variance of ITF, indicating certain meridian specificity exists. It could be one proof for the TCM theory “Relative particularity between SMFY and stomach”.
Mesh Headings (Keywords): Acupuncture Points, Animals, Electroacupuncture, Female, Gastric Mucosa, Gene Expression Regulation, Male, Peptides, RNA, Messenger, Rats, Rats, Sprague-Dawley, Stress
Check for Full Text / PubMed Unique Identifier (PMID): 16610008
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