Postnatal Expression of Klf12 in the Inner Medullary Collecting Ducts of Kidney and Its Trans-activation of Ut-a1 Urea Transporter Promoter.
From: Department of Nephrology, Graduate School of Medicine, Tokyo Medical and Dental University, Japan.
Biochemical and biophysical research communications
- Publish Date: May 2006
- ISSN: 0006-291X
- Volume: 344
- Issue: 1
- Pages: 246-52
- Medium: Print
- Language: English
- Citation (JAMA): Suda Shin, Rai Tatemitsu, Sohara Eisei, et al. Postnatal Expression of Klf12 in the Inner Medullary Collecting Ducts of Kidney and Its Trans-activation of Ut-a1 Urea Transporter Promoter.. Biochem. Biophys. Res. Commun. May 2006;344:246-52
Abstract
Maturation of the inner medulla of the kidney occurs after birth and is vital for mammals to acquire maximal urinary concentrating ability. During this process, expression of several kidney transporters and channels involved in urine concentrating mechanisms is known to be regulated. We previously isolated KLF15 as a transcription factor that regulates the expression of the ClC-K1 chloride channel. We have now found that another KLF transcription factor, KLF12, is expressed in the kidney from around 15 days after birth. To gain insight into its involvement in the maturation process of the inner medulla, we first determined the expression site of KLF12 within the kidney by in situ hybridization. By comparing the AQP2 immunolocalization in sequential sections, KLF12 was found to be expressed in the collecting ducts. Because expression of the urea transporter UT-A1 and amiloride-sensitive epithelial sodium channels ENaC is known to be tightly regulated in the collecting ducts after birth, we tested whether KLF12 has a regulatory role in the promoter activities of these genes. KLF12 is able to increase UT-A1 but not ENaC promoter activity through the binding to CACCC motif. These results suggest that KLF12 is involved in the maturation processes of collecting ducts after birth, and that UT-A1 is a target gene of KLF12.
Mesh Headings (Keywords): Animals, DNA-Binding Proteins, Dogs, Electrophoretic Mobility Shift Assay, In Situ Hybridization, Kidney Medulla, Kidney Tubules, Collecting, Kruppel-Like Transcription Factors, Membrane Transport Proteins, Mice, Mice, Inbred C57BL, Promoter Regions (Genetics), RNA, Messenger, Trans-Activation (Genetics), Trans-Activators, Transcription Factors
Check for Full Text / PubMed Unique Identifier (PMID): 16615998
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