Stable Rna Interference of Host Thrombospondin-1 Blocks Trypanosoma Cruzi Infection.
From: Division of Microbial Pathogenesis and Immune Response, Department of Biomedical Sciences, School of Medicine, Meharry Medical College, Nashville, TN 37208, USA.
FEBS letters
- Publish Date: Apr 2006
- ISSN: 0014-5793
- Volume: 580
- Issue: 9
- Pages: 2365-70
- Medium: Print
- Language: English
- Citation (JAMA): Simmons Kaneatra J, Nde Pius N, Kleshchenko Yuliya Y, et al. Stable Rna Interference of Host Thrombospondin-1 Blocks Trypanosoma Cruzi Infection.. FEBS Lett. Apr 2006;580:2365-70
Abstract
Interactions between Trypanosoma cruzi and the extracellular matrix play an important role in cellular invasion. Here we show that T. cruzi increases the levels of thrombospondin-1 (TSP-1) expression in host cells during early infection. Stable RNA interference of host cell TSP-1 knocks down the levels of TSP-1 transcripts and protein expression in mammalian cells causing inhibition of T. cruzi infection. Addition of TSP-1 to these cells restores infection. Thus, host TSP-1, regulated by the parasite, plays a crucial role in early infection. This is the first report showing that a human parasite modulates TSP-1 expression to facilitate infection.
Mesh Headings (Keywords): Animals, Cells, Cultured, Chagas Disease, Humans, RNA Interference, Thrombospondin 1, Trypanosoma cruzi
Check for Full Text / PubMed Unique Identifier (PMID): 16616140
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