Specific Phospholipid Recognition by Human Immunodeficiency Virus Type-1 Neutralizing Anti-gp41 2f5 Antibody.
From: Biofisika Unitatea (CSIC-UPV/EHU) and Biokimika Saila, Euskal Herriko Unibertsitatea, Posta Kutxa 644, 48080 Bilbao, Spain.
FEBS letters
- Publish Date: Apr 2006
- ISSN: 0014-5793
- Volume: 580
- Issue: 9
- Pages: 2395-99
- Medium: Print
- Language: English
- Citation (JAMA): Sánchez-Martínez Silvia, Lorizate Maier, Hermann Katinger, et al. Specific Phospholipid Recognition by Human Immunodeficiency Virus Type-1 Neutralizing Anti-gp41 2f5 Antibody.. FEBS Lett. Apr 2006;580:2395-99
Abstract
HIV-1 neutralizing monoclonal antibody (Mab) 2F5 recognizes a membrane-partitioning gp41 sequence. Just recently its capacity to react with cardiolipin has been demonstrated. Here, we have studied the specificity of Mab2F5-phospholipid interactions comparing partitioning into lipid bilayers with recognition of molecular species dispersed in solution. Using a liposome-based ELISA we demonstrate a preferential association with cardiolipin bilayers. When different soluble lysoderivatives were compared in their capacity to inhibit Mab2F5 binding to immobilized HIV-1 peptide epitope, only dilysocardiolipin resulted effective in blocking the process. Dilyso-cardiolipin also competed with native-functional gp41 for 2F5 recognition. Thus, our data support specific cardiolipin recognition by 2F5 that is not dependent on lipid bilayer assembly and involves the epitope-binding site. These findings might be of relevance for understanding the molecular basis of HIV-1 immune evasion.
Mesh Headings (Keywords): Animals, Antibodies, Monoclonal, Antibodies, Viral, Binding Sites, Antibody, Cardiolipins, Epitope Mapping, Epitopes, HIV Envelope Protein gp41, HIV-1, Humans, Lipid Bilayers, Membrane Fusion, Peptides
Check for Full Text / PubMed Unique Identifier (PMID): 16616522
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.