Piperine Inhibition of 1-methyl-4-phenylpyridinium-induced Mitochondrial Dysfunction and Cell Death in Pc12 Cells.
From: Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul 156-756, South Korea. leecs@cau.ac.kr
European journal of pharmacology
- Publish Date: May 2006
- ISSN: 0014-2999
- Volume: 537
- Issue: 1-3
- Pages: 37-44
- Medium: Print
- Language: English
- Citation (JAMA): Lee Chung Soo, Han Eun Sook, Kim Young Ki, et al. Piperine Inhibition of 1-methyl-4-phenylpyridinium-induced Mitochondrial Dysfunction and Cell Death in Pc12 Cells.. Eur. J. Pharmacol. May 2006;537:37-44
Abstract
The effect of alkaloid piperine against the toxicity of 1-methyl-4-phenylpyridinium (MPP(+)) in differentiated PC12 cells was assessed. Piperine treatment revealed a differential effect on the cytotoxicity of MPP(+) and had its maximum inhibitory effect at 1 microM. The addition of piperine (0.5-10 microM) significantly reduced the MPP(+)-induced nuclear damage, mitochondrial membrane permeability changes, formation of reactive oxygen species and depletion of GSH. In contrast, piperine at 50-100 microM showed cytotoxicity and exhibited an additive effect against the MPP(+) toxicity. The results indicate that piperine had a differential effect on the cytotoxicity of MPP(+) depending on concentration. Piperine at low concentrations may reduce the MPP(+)-induced viability loss in PC12 cells by suppressing the changes in the mitochondrial membrane permeability, leading to the release of cytochrome c and subsequent activation of caspase-3. The effects may be ascribed to its inhibitory action on the formation of reactive oxygen species and depletion of GSH.
Mesh Headings (Keywords): 1-Methyl-4-phenylpyridinium, Alkaloids, Animals, Benzodioxoles, Caspase 3, Caspases, Cell Death, Cell Membrane Permeability, Cytochromes c, DNA Fragmentation, Glutathione, Lipid Peroxidation, Malondialdehyde, Membrane Potentials, Mitochondria, Mitochondrial Membranes, PC12 Cells, Piperidines, Polyunsaturated Alkamides, Rats, Reactive Oxygen Species, Thiobarbituric Acid Reactive Substances
Check for Full Text / PubMed Unique Identifier (PMID): 16624279
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.