Differential Analysis of D-beta-asp-containing Proteins Found in Normal and Infrared Irradiated Rabbit Lens.
From: Research Reactor Institute, Kyoto University, Kumatori-cho, Sennan, Osaka 590-0494, Japan.
Biochemical and biophysical research communications
- Publish Date: May 2006
- ISSN: 0006-291X
- Volume: 344
- Issue: 1
- Pages: 263-71
- Medium: Print
- Language: English
- Citation (JAMA): Takata Takumi, Shimo-Oka Tadashi, Kojima Masami, et al. Differential Analysis of D-beta-asp-containing Proteins Found in Normal and Infrared Irradiated Rabbit Lens.. Biochem. Biophys. Res. Commun. May 2006;344:263-71
Abstract
Although proteins are generally composed of l-alpha-amino acids, d-beta-aspartic acid (Asp)-containing proteins have been reported in various elderly tissues. Our previous study detected several d-beta-Asp-containing proteins in a rabbit lens derived from epithelial cell line by Western blot analysis of a 2D-gel using a polyclonal antibody that is highly specific for d-beta-Asp-containing proteins. The identity of each spot was subsequently determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and the Ms-Fit online database searching algorithm. In this study, we discovered novel d-beta-Asp-containing proteins from rabbit lens. The results indicate that beta-crystallin A3, beta-crystallin A4, beta-crystallin B1, beta-crystallin B2, beta-crystallin B3, gamma-crystallin C, gamma-crystallin D, and lambda-crystallin in rabbit lens contain d-beta-Asp residues. Furthermore, the occurrence of d-beta-Asp residues increases with infrared ray (IR) irradiation. Additionally, some d-beta-Asp-containing proteins only appear after IR irradiation. One such protein is the alpha-enolase, which shows homology to tau-crystallin.
Mesh Headings (Keywords): Animals, Electrophoresis, Gel, Two-Dimensional, Infrared Rays, Isoaspartic Acid, Lens, Crystalline, Rabbits, Solubility, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, beta-Crystallins
Check for Full Text / PubMed Unique Identifier (PMID): 16630575
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