Tissue Inhibitors of Metalloproteinases-1 (Timp-1) and -2(Timp-2) Are Major Serum Factors That Stimulate the Timp-1 Gene in Human Gingival Fibroblasts.
From: Department of Biochemistry, School of Dentistry, Aichi-Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya 464-8650, Japan.
Biochimica et biophysica acta
- Publish Date: Mar 2006
- ISSN: 0006-3002
- Volume: 1763
- Issue: 3
- Pages: 296-304
- Medium: Print
- Language: English
- Citation (JAMA): Guo Xiao-Kui, Zhao Wan-Qian, Kondo Chihiro, et al. Tissue Inhibitors of Metalloproteinases-1 (Timp-1) and -2(Timp-2) Are Major Serum Factors That Stimulate the Timp-1 Gene in Human Gingival Fibroblasts.. Biochim. Biophys. Acta Mar 2006;1763:296-304
Abstract
We demonstrate in this study that both TIMP-1 and TIMP-2 are major serum factors that stimulate the induction of TIMP-1 mRNA in quiescent human gingival fibroblasts (Gin-1 cells) at mid-G1 (6-9 h after serum stimulation) of the cell cycle, but not that of TIMP-2. When we chased the secretion of both TIMP proteins into culture medium containing 10% FCS freed of both TIMPs, TIMP-2 secretion rose to the level in 10% FCS after 24 h, but TIMP-1 secretion remained at a fairly low level even after 3 days, thus reflecting a contrastive difference in the induction of both TIMP mRNAs. The stimulating activity of TIMP-1 on the expression of the TIMP-1 gene switched over to inhibitory activity, when the TIMP-1 concentration in the culture medium exceeded about 30 ng/ml. The depletion of TIMP-1 and TIMP-2 from FCS affected remarkably the induction of c-jun and c-fos mRNAs, but not that of c-ets-1 mRNA. TIMP-1 and TIMP-2-dependent expression of AP-1 protein was further demonstrated by using nuclear extracts of Gin-1 cells in an electrophoretic mobility shift assay.
Mesh Headings (Keywords): Binding Sites, Cells, Cultured, Culture Media, Dactinomycin, Fibroblasts, Gene Expression Regulation, Gingiva, Humans, Proto-Oncogene Protein c-ets-1, Proto-Oncogene Proteins c-fos, Proto-Oncogene Proteins c-jun, Puromycin, RNA, Messenger, Time Factors, Tissue Inhibitor of Metalloproteinase-1, Tissue Inhibitor of Metalloproteinase-2, Transcription Factor AP-1
Check for Full Text / PubMed Unique Identifier (PMID): 16631927
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