Functional Genomics of Ppar-gamma in Human Immunomodulatory Cells.
From: Molecular Biology Unit, Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research, Chandigarh 160012, India. dkaul_24@hotmail.com
Molecular and cellular biochemistry
- Publish Date: Oct 2006
- ISSN: 0300-8177
- Volume: 290
- Issue: 1-2
- Pages: 211-5
- Medium: Print
- Language: English
- Citation (JAMA): Kaul Deepak, Anand Paras K, Khanna Amit, et al. Functional Genomics of Ppar-gamma in Human Immunomodulatory Cells.. Mol. Cell. Biochem. Oct 2006;290:211-5
Abstract
Keeping in view the fact that peroxisome-proliferators activated receptors-PPARs (alpha,gamma) play a crucial role in atherogenic inflammation, the present study was addressed to explore as to how selective and specific PPAR-gamma gene silencing within human mononuclear cells affects genes involved in lipid metabolism and innate immune process. Such a study revealed that with respect to control cells, the PPAR-gamma knock-out cells exhibited significant reduction in the expression of genes coding for PPAR- alpha, CD-36, LDL-R as well as significant increase in the expression of genes coding for IL-4, IL-8, IFN-gamma, CX3CR1, hTERT. However, the expression of genes coding for LXR-alpha and Receptor-C( k ) could not be detected in PPAR-gamma knock-out cells. Based on these results, we propose that PPAR-gamma gene has the inherent capacity to influence the lipid mediated inflammation process in atherosclerotic lesions.
Mesh Headings (Keywords): Cells, Cultured, Genomics, Humans, Immunity, Natural, Inflammation, Lipid Metabolism, PPAR gamma, RNA, Messenger, RNA, Small Interfering, Transfection
Check for Full Text / PubMed Unique Identifier (PMID): 16633734
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