Down-regulation of Drebrin A Expression Suppresses Synaptic Targeting of Nmda Receptors in Developing Hippocampal Neurones.
From: Department of Neurobiology and Behavior, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
Journal of neurochemistry
- Publish Date: Apr 2006
- ISSN: 0022-3042
- Volume: 97 Suppl 1
- Issue:
- Pages: 110-5
- Medium: Print
- Language: English
- Citation (JAMA): Takahashi Hideto, Mizui Toshiyuki, Shirao Tomoaki, et al. Down-regulation of Drebrin A Expression Suppresses Synaptic Targeting of Nmda Receptors in Developing Hippocampal Neurones.. J. Neurochem. Apr 2006;97 Suppl 1:110-5
Abstract
Drebrin is a major F-actin-binding protein in the brain. We have recently demonstrated that drebrin A (neurone-specific isoform) clusters at synapses and governs targeting of the post-synaptic density 95 protein to synapses during development. To determine the role of drebrin A on excitatory synapse formation, we analysed whether the suppression of drebrin A expression affects filopodia-spine morphology and synaptic targeting of NMDA receptors in cultured hippocampal neurones. Suppression of developmentally programmed up-regulation of drebrin A by antisense treatment significantly decreased the density and width of filopodia-spines. Immunocytochemistry showed that the antisense treatment did not attenuate synaptic clustering of NMDA receptors under conditions that permitted spontaneous activities but inhibited the accelerated targeting of NMDA receptors into synapses by its antagonist D-(-)-2-amino-5-phosphonopentanoic acid. These results indicate that drebrin A up-regulation plays a pivotal role in spine morphogenesis and activity-dependent synaptic targeting of NMDA receptors.
Mesh Headings (Keywords): Cells, Cultured, Dendrites, Gene Expression Regulation, Hippocampus, Neurons, Neuropeptides, Oligonucleotides, Antisense, Receptors, N-Methyl-D-Aspartate, Synapses, Synapsins
Check for Full Text / PubMed Unique Identifier (PMID): 16635259
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.