Effect of Desferrioxamine, a Strong Iron (Iii) Chelator, on 1-methyl-4-phenylpyridinium Ion (Mpp+)-induced Hydroxyl Radical Generation in the Rat Striatum.
From: Department of Analytical Chemistry, Ohu University School of Pharmaceutical Sciences, Koriyama, Fukushima 963-8611, Japan. t-obata@pha.ohu-u.ac.jp
European journal of pharmacology
- Publish Date: Jun 2006
- ISSN: 0014-2999
- Volume: 539
- Issue: 1-2
- Pages: 34-8
- Medium: Print
- Language: English
- Citation (JAMA): Obata Toshio, et al. Effect of Desferrioxamine, a Strong Iron (Iii) Chelator, on 1-methyl-4-phenylpyridinium Ion (Mpp+)-induced Hydroxyl Radical Generation in the Rat Striatum.. Eur. J. Pharmacol. Jun 2006;539:34-8
Abstract
The present study was examined that the desferrioxamine, a strong iron (III) chelator, enhanced 1 methyl-4-phenylpyridinium ion (MPP+)-induced hydroxyl radical (*OH) generation in the extracellular fluid of caudate nucleus anesthetized rats. Rats were anesthetized, and sodium salicylate in Ringer’s solution (0.5 nmol/microl/min) was infused through a microdialysis probe to detect the generation of *OH as reflected by the non-enzymatic formation of 2,3-dihydroxybenzoic acid (DHBA) in the striatum. Induction of desferrioxamine (50 microM) drastically increased the formation of *OH trapped as 2,3-DHBA by the action of MPP+, as compared with MPP+-only-treated animals. Although desferrioxamine did not change the levels of MPP+-induced dopamine, a marked elevation of *OH formation trapped as 2,3-DHBA was observed. When corresponding experiments were performed with reserpinized animals, the level of dopamine and 2,3-DHBA drastically decreased. However, the level of dopamine did not change, but desferrioxamine significantly increased the level of 2,3-DHBA in reserpinized animals. Iron (III) decreased MPP+-induced 2,3-DHBA formations in the presence of dopamine (10 microM). Moreover, when iron (II) was administered to desferrioxamine-treated animals, a marked elevation of 2,3-DHBA was observed, compared with MPP+-only-treated animals, that showed a positive linear correlation between iron (II) and *OH formation trapped as 2,3-DHBA (R2=0.981) in the dialysate. The present study indicates that the suppression of MPP+-induced *OH formation by iron (III) may play a key role in protective effect of iron (III) on the rat brain.
Mesh Headings (Keywords): 1-Methyl-4-phenylpyridinium, Animals, Cations, Corpus Striatum, Deferoxamine, Dopamine, Extracellular Space, Ferric Compounds, Hydroxyl Radical, Iron Chelating Agents, Male, Microdialysis, Rats, Rats, Wistar
Check for Full Text / PubMed Unique Identifier (PMID): 16650845
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