Fast Micromethod Dna Single-strand-break Assay.
From: Abteilung Angewandte Molekularbiologie, Institut für Physiologische Chemie, Universität, Mainz, Germany.
Methods in molecular biology (Clifton, N.J.)
- Publish Date: 2006
- ISSN: 1064-3745
- Volume: 314
- Issue:
- Pages: 287-305
- Medium: Print
- Language: English
- Citation (JAMA): Schröder Heinz C, Batel Renato, Schwertner Heiko, et al. Fast Micromethod Dna Single-strand-break Assay.. Methods Mol. Biol. 2006;314:287-305
Abstract
The Fast Micromethod is a convenient and quick fluorimetric microplate assay for the assessment of DNA single-strand breaks and their repair. This method measures the rate of unwinding of cellular DNA on exposure to alkaline conditions using a fluorescent dye which preferentially binds to double-stranded DNA, but not to single-stranded DNA or protein. The advantages of this method are that it requires only minute amounts of material (30 ng of DNA or about 3000 cells per single well), it allows simultaneous measurements of multiple samples, and it can be performed within 3 h or less (for one 96-well microplate). The Fast Micromethod can be used for the routine determination of DNA damage in cells and tissue samples after irradiation, exposure to mutagenic and carcinogenic agents, or chemotherapy.
Mesh Headings (Keywords): Animals, Cattle, Cells, Cultured, DNA, DNA Damage, DNA, Single-Stranded, Humans, Leukocytes, Mononuclear, Male, Mice, Microchemistry, Spectrometry, Fluorescence, Spermatozoa
Check for Full Text / PubMed Unique Identifier (PMID): 16673889
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