Characterization of Enzymes That Initiate Base Excision Repair at Abasic Sites.
From: Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, LA, USA.
Methods in molecular biology (Clifton, N.J.)
- Publish Date: 2006
- ISSN: 1064-3745
- Volume: 314
- Issue:
- Pages: 355-64
- Medium: Print
- Language: English
- Citation (JAMA): Deutsch Walter A, Hegde Vijay, et al. Characterization of Enzymes That Initiate Base Excision Repair at Abasic Sites.. Methods Mol. Biol. 2006;314:355-64
Abstract
Abasic sites in DNA arise under a variety of circumstances, including destabilization of bases through oxidative stress, as an intermediate in base excision repair, and through spontaneous loss. Their persistence can yield a blockade to RNA transcription and DNA synthesis and can be a source of mutations. Organisms have developed an enzymatic means of repairing abasic sites in DNA that generally involves a DNA repair pathway that is initiated by a repair protein creating a phosphodiester break (“nick”) adjacent to the site of base loss. Here we describe a method for analyzing the manner in which repair endonucleases differ in the way they create nicks in DNA and how to distinguish between them using cellular crude extracts.
Mesh Headings (Keywords): Alkalies, Animals, Apurinic Acid, DNA, DNA Repair, DNA-(Apurinic or Apyrimidinic Site) Lyase, Electrophoresis, Humans, Isotope Labeling, Nucleic Acid Denaturation, Oligonucleotides, Polynucleotides, Uracil
Check for Full Text / PubMed Unique Identifier (PMID): 16673893
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