Intranigral Transplants of Immortalized Gabaergic Cells Decrease the Expression of Kainic Acid-induced Seizures in the Rat.
From: Department of Cognitive and Behavioural Neurobiology, Instituto de Neurobiología, Campus Juriquilla, Universidad Nacional Autónoma de México, Querétaro.
Behavioural brain research
- Publish Date: Jul 2006
- ISSN: 0166-4328
- Volume: 171
- Issue: 1
- Pages: 109-15
- Medium: Print
- Language: English
- Citation (JAMA): Castillo Claudia G, Mendoza Soledad, Freed William J, et al. Intranigral Transplants of Immortalized Gabaergic Cells Decrease the Expression of Kainic Acid-induced Seizures in the Rat.. Behav. Brain Res. Jul 2006;171:109-15
Abstract
Repeated systemic administration of low doses of kainic acid (KA) induces spontaneous convulsive seizures [Hellier JL, Patrylo PR, Buckmaster PS, Dudek FE. Recurrent spontaneous motor seizures after repeated low-dose systemic treatment with kainate: assessment of a rat model of temporal lobe epilepsy. Epilepsy Res 1998;31:73-84]. In this study, male Sprague-Dawley animals received intranigral transplants of a control cell line M213-2O, or a cell line transfected with human GAD67 cDNA (M213-2O CL4) [Conejero-Goldberg C, Tornatore C, Abi-Saab W, Monaco MC, Dillon-Carter O, Vawter M, et al. Transduction of human GAD67 cDNA into immortalized striatal cell lines using an Epstein-Barr virus-based plasmid vector increases GABA content. Exp Neurol 2000;161:453-61], or no transplant. Eight weeks after transplantation surgery, KA was administered (5 mg/kg/h) until animals reached stage V seizures as described by Racine [Racine RJ. Modification of seizure activity by electrical stimulation. II. Motor seizure. Electroencephalogr Clin Neurophysiol 1972;32:281-94]. The group transplanted with CL4 required a larger dose of KA and a longer latency to reach a stage V seizure. In addition, this group exhibited significantly fewer stage III and IV seizures. These results indicate that intranigral transplants of a GABA-producing cell line can decrease the number of kainic acid-induced seizures.
Mesh Headings (Keywords): Animals, Cell Line, Transformed, Glutamate Decarboxylase, Humans, Isoenzymes, Kainic Acid, Male, Neostriatum, Neurons, Rats, Rats, Sprague-Dawley, Seizures, Substantia Nigra, Transgenes, gamma-Aminobutyric Acid
Check for Full Text / PubMed Unique Identifier (PMID): 16677720
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.