Rapid and Sensitive Fame Analysis of Bacteria by Cold Trap Injection Gas Chromatography.
From: ARS, SASL, US Department of Agriculture, Building 001 Room 140 BARC-West, Beltsville, MD 20705-2350, USA. buyerj@ba.ars.usda.gov
Journal of microbiological methods
- Publish Date: Oct 2006
- ISSN: 0167-7012
- Volume: 67
- Issue: 1
- Pages: 187-90
- Medium: Print
- Language: English
- Citation (JAMA): Buyer Jeffrey S, et al. Rapid and Sensitive Fame Analysis of Bacteria by Cold Trap Injection Gas Chromatography.. J. Microbiol. Methods Oct 2006;67:187-90
Abstract
Whole cell fatty acid analysis is commonly used to identify bacteria. A cold trap, using a commercially available device that directs a stream of compressed air across a section of the GC column, is used to focus peaks at the head of the column. When combined with a rapid sample processing method that uses smaller volumes of solvents, it becomes possible to correctly identify bacteria from 1 to 2 mg of biomass.
Mesh Headings (Keywords): Bacteria, Chromatography, Gas, Fatty Acids, Sensitivity and Specificity
Check for Full Text / PubMed Unique Identifier (PMID): 16678289
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