Promotion of Hair Follicle Development and Trichogenesis by Wnt-10b in Cultured Embryonic Skin and in Reconstituted Skin.
From: Program in Tissue Engineering and Department of Parasitology, Nara Medical University, Kashihara, Nara, Japan. oujix@naramed-u.ac.jp
Biochemical and biophysical research communications
- Publish Date: Jun 2006
- ISSN: 0006-291X
- Volume: 345
- Issue: 2
- Pages: 581-7
- Medium: Print
- Language: English
- Citation (JAMA): Ouji Yukiteru, Yoshikawa Masahide, Shiroi Akira, et al. Promotion of Hair Follicle Development and Trichogenesis by Wnt-10b in Cultured Embryonic Skin and in Reconstituted Skin.. Biochem. Biophys. Res. Commun. Jun 2006;345:581-7
Abstract
We previously showed that Wnt-10b promoted the differentiation of primary skin epithelial cells (MPSEC) toward hair shaft and inner root sheath of the hair follicle (IRS) cells in vitro. In the present study, we found that Wnt-10b promotes the development of hair follicles using a culture of mouse embryonic skin tissue and trichogenesis using a reconstitution experiment with nude mice. Hair follicle development was observed in skin taken from mouse embryos on embryonic day 10.5 following a 2-day culture with recombinant Wnt-10b (rWnt-10b), however, not without rWnt-10b. Brown hair growth was observed at the site of reconstituted skin in Balb/c nude mice where dermal fibroblasts and keratinocytes, derived from C3H/HeN new born mice, were transplanted with Wnt-10b-producing COS cells (Wnt-COS). Without the co-transplantation of Wnt-COS, no hair growth was observed. Our results suggest an important role of Wnt-10b in the initiation of hair follicle development and following trichogenesis.
Mesh Headings (Keywords): Animals, COS Cells, Cell Proliferation, Cell Transplantation, Cells, Cultured, Cercopithecus aethiops, Fibroblasts, Hair Follicle, Keratinocytes, Mice, Mice, Inbred BALB C, Mice, Nude, Proto-Oncogene Proteins, Skin, Wnt Proteins
Check for Full Text / PubMed Unique Identifier (PMID): 16690019
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