Caveolin-1 is Required for Fatty Acid Translocase (Fat/Cd36) Localization and Function at the Plasma Membrane of Mouse Embryonic Fibroblasts.
From: Department of Internal Medicine IV, Ruprechts-Karls-University Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
Biochimica et biophysica acta
- Publish Date: Apr 2006
- ISSN: 0006-3002
- Volume: 1761
- Issue: 4
- Pages: 416-23
- Medium: Print
- Language: English
- Citation (JAMA): Ring Axel, Le Lay Soazig, Pohl Juergen, et al. Caveolin-1 is Required for Fatty Acid Translocase (Fat/Cd36) Localization and Function at the Plasma Membrane of Mouse Embryonic Fibroblasts.. Biochim. Biophys. Acta Apr 2006;1761:416-23
Abstract
Several lines of evidence suggest that lipid rafts are involved in cellular fatty acid uptake and influence fatty acid translocase (FAT/CD36) function. However, it remains unknown whether caveolae, a specialized raft type, are required for this mechanism. Here, we show that wild-type (WT) mouse embryonic fibroblasts (MEFs) and caveolin-1 knockout (KO) MEFs, which are devoid of caveolae, have comparable overall expression of FAT/CD36 protein but altered subcellular FAT/CD36 localization and function. In WT MEFs, FAT/CD36 was isolated with both lipid raft enriched detergent-resistant membranes (DRMs) and detergent-soluble membranes (DSMs), whereas in cav-1 KO cells it was exclusively associated with DSMs. Subcellular fractionation demonstrated that FAT/CD36 in WT MEFs was localized intracellularly and at the plasma membrane level while in cav-1 KO MEFs it was absent from the plasma membrane. This mistargeting of FAT/CD36 in cav-1 KO cells resulted in reduced fatty acid uptake compared to WT controls. Adenoviral expression of caveolin-1 in KO MEFs induced caveolae formation, redirection of FAT/CD36 to the plasma membrane and rescue of fatty acid uptake. In conclusion, our data provide evidence that caveolin-1 is necessary to target FAT/CD36 to the plasma membrane. Caveolin-1 may influence fatty acid uptake by regulating surface availability of FAT/CD36.
Mesh Headings (Keywords): Animals, Antigens, CD36, Caveolae, Caveolin 1, Cell Membrane, Cells, Cultured, Fatty Acids, Fibroblasts, Mice, Mice, Knockout, Oleic Acids, Subcellular Fractions, Succinimides
Check for Full Text / PubMed Unique Identifier (PMID): 16702023
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