Regulation of Ecto-5'-nucleotidase by Nacl and Nitric Oxide: Potential Roles in Tubuloglomerular Feedback and Adaptation.
From: Div. of Nephrology-Hypertension, UC San Diego and VASDHS, 3350 La Jolla Village Dr. (9111-H San Diego, CA 92161, USA.
American journal of physiology. Renal physiology
- Publish Date: Nov 2006
- ISSN: 0363-6127
- Volume: 291
- Issue: 5
- Pages: F1078-82
- Medium: Print
- Language: English
- Citation (JAMA): Satriano Joseph, Wead Lucinda, Cardus Anna, et al. Regulation of Ecto-5'-nucleotidase by Nacl and Nitric Oxide: Potential Roles in Tubuloglomerular Feedback and Adaptation.. Am. J. Physiol. Renal Physiol. Nov 2006;291:F1078-82
Abstract
The tubuloglomerular feedback (TGF) system serves to establish an appropriate balance between tubular reabsorption and glomerular filtration rate (GFR). High salt at the macula densa activates TGF to decrease GFR. Effector molecules for the TGF signal include ATP and adenosine. Over time, the GFR will adapt by increasing even if a high salt concentration persists. A potential modulator of this TGF adaptation is nitric oxide synthase-1-derived nitric oxide (NO). In isolated glomerular preparations, we developed a system for evaluating the effects of changing dietary salt on ecto-5’-nucleotidase (ecto-5’-NT) activity, the final enzyme in the conversion of ATP to adenosine. We found observable ecto-5’-NT activity in isolated glomeruli and that this activity can be regulated by dietary salt, with high salt increasing activity. Conversely, NO decreases ecto-5’-NT activity in glomerular preparations. Moreover, NO inhibition of ecto-5’-NT activity is suppressed in the presence of dithiothreitol, suggesting nitrosylation as a reversible, oxidative stress-sensitive mechanism. The salt-induced activation of ecto-5’-NT correlates with high salt resetting of TGF. NO inhibition of enzymatic activity could be part of the adaptive phase.
Mesh Headings (Keywords): 5’-Nucleotidase, Adaptation, Physiological, Adenosine, Animals, Enzyme Activation, Feedback, Biochemical, Glomerular Filtration Rate, Kidney Glomerulus, Kidney Tubules, Male, Nitric Oxide, Nitric Oxide Donors, Nitric Oxide Synthase, Nitrogen Oxides, Rats, Rats, Wistar, Sodium Chloride, Spermine
Check for Full Text / PubMed Unique Identifier (PMID): 16705150
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