Chlamydial Dna Polymerase I Can Bypass Lesions in Vitro.
From: College of Life Sciences and Technology, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, China.
Biochemical and biophysical research communications
- Publish Date: Jul 2006
- ISSN: 0006-291X
- Volume: 345
- Issue: 3
- Pages: 1083-91
- Medium: Print
- Language: English
- Citation (JAMA): Liu Xipeng, Hou Jingli, Liu Jianhua, et al. Chlamydial Dna Polymerase I Can Bypass Lesions in Vitro.. Biochem. Biophys. Res. Commun. Jul 2006;345:1083-91
Abstract
We found that DNA polymerase I from Chlamydiophila pneumoniae AR39 (CpDNApolI) presents DNA-dependent DNA polymerase activity, but has no detectable 3’ exonuclease activity. CpDNApolI-dependent DNA synthesis was performed using DNA templates carrying different lesions. DNAs containing 2’-deoxyuridine (dU), 2’-deoxyinosine (dI) or 2’-deoxy-8-oxo-guanosine (8-oxo-dG) served as templates as effectively as unmodified DNAs for CpDNApolI. Furthermore, the CpDNApolI could bypass natural apurinic/apyrimidinic sites (AP sites), deoxyribose (dR), and synthetic AP site tetrahydrofuran (THF). CpDNApolI could incorporate any dNMPs opposite both of dR and THF with the preference to dAMP-residue. CpDNApolI preferentially extended primer with 3’-dAMP opposite dR during DNA synthesis, however all four primers with various 3’-end nucleosides (dA, dT, dC, and dG) opposite THF could be extended by CpDNApolI. Efficiently bypassing of AP sites by CpDNApolI was hypothetically attributed to lack of 3’ exonuclease activity.
Mesh Headings (Keywords): Amino Acid Motifs, Amino Acid Sequence, Base Sequence, Binding Sites, Chlamydophila pneumoniae, DNA, DNA Damage, DNA Polymerase I, DNA Primers, Exonucleases, Kinetics, Molecular Sequence Data, Recombinant Proteins, Sequence Homology, Nucleic Acid
Check for Full Text / PubMed Unique Identifier (PMID): 16712785
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.