The Cell-mediated Immune Response Induced by Plasmid Encoding Bovine Herpesvirus 1 Glycoprotein B is Enhanced by Plasmid Encoding Il-12 when Delivered Intramuscularly or by Gene Gun, but Not After Intradermal Injection.
From: Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Rd., Saskatoon, Saskatchewan, Canada S7N 5E3.
Vaccine
- Publish Date: Jun 2006
- ISSN: 0264-410X
- Volume: 24
- Issue: 25
- Pages: 5349-59
- Medium: Print
- Language: English
- Citation (JAMA): Huang Y, Babiuk L A, van Drunen Littel-van den Hurk S, et al. The Cell-mediated Immune Response Induced by Plasmid Encoding Bovine Herpesvirus 1 Glycoprotein B is Enhanced by Plasmid Encoding Il-12 when Delivered Intramuscularly or by Gene Gun, but Not After Intradermal Injection.. Vaccine Jun 2006;24:5349-59
Abstract
Bovine herpesvirus 1 (BHV-1) causes respiratory and genital infections in cattle. Previously we demonstrated that a DNA vaccine encoding a truncated, secreted form of BHV-1 glycoprotein B (tgB) induces cytotoxic T lymphocyte (CTL) responses in C3H mice. In this study we investigated the potential of interleukin 12 (IL-12) to further enhance the CTL response. C3H mice were immunized with a plasmid encoding tgB or with plasmids encoding tgB and murine IL-12. When the plasmid encoding tgB was delivered intramuscularly or epidermally by a gene gun, co-administration with IL-12 plasmid stimulated the synthesis of more IgG2a, the production of higher levels of IFN-gamma, and more effective killing by CTLs. In contrast, after intradermal delivery no effect of co-administration of IL-12 encoding plasmid was observed. Further investigation suggested that antigen and IL-12 need to be expressed in the draining lymph nodes, where IL-12 can have a direct effect on T cells.
Mesh Headings (Keywords): Animals, Antibodies, Viral, Biolistics, COS Cells, Cattle, Cercopithecus aethiops, Herpesvirus 1, Bovine, Herpesvirus Vaccines, Humans, Immunization, Injections, Intradermal, Injections, Intramuscular, Interleukin-12, Mice, Mice, Inbred C3H, Plasmids, T-Lymphocytes, Cytotoxic, Vaccines, DNA, Viral Envelope Proteins
Check for Full Text / PubMed Unique Identifier (PMID): 16714071
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