Medical Journals

Three-dimensional Imaging of Monogenoidean Sclerites by Laser Scanning Confocal Fluorescence Microscopy.

Authors:
  • Galli Paolo
  • Strona Giovanni
  • Villa Anna Maria
  • Benzoni Francesca
  • Fabrizio Stefani
  • Doglia Silvia Maria
  • Kritsky Delane C

From: Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.

The Journal of parasitology

  • Publish Date: Apr 2006
  • ISSN: 0022-3395
  • Volume: 92
  • Issue: 2
  • Pages: 395-9
  • Medium: Print
  • Language: English
  • Citation (JAMA): Galli Paolo, Strona Giovanni, Villa Anna Maria, et al. Three-dimensional Imaging of Monogenoidean Sclerites by Laser Scanning Confocal Fluorescence Microscopy.. J. Parasitol. Apr 2006;92:395-9

Abstract

A nondestructive protocol for preparing specimens of Monogenoidea for both alpha-taxonomic studies and reconstruction of 3-dimensional structure is presented. Gomori’s trichrome, a stain commonly used to prepare whole-mount specimens of monogenoids for taxonomic purposes, is used to provide fluorescence of genital spines, the copulatory organ, accessory piece, squamodisc, anchors, hooks, bars, and clamps under laser scanning confocal microscopy.

Mesh Headings (Keywords): Animals, Carps, Fish Diseases, Microscopy, Confocal, Perciformes, Platyhelminths, Trematode Infections


Check for Full Text / PubMed Unique Identifier (PMID): 16729702


This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.

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