Rhoc is Essential for Tgf-beta1-induced Invasive Capacity of Rat Ascites Hepatoma Cells.
From: Department of Biochemistry, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka 537-8511, Japan.
Biochemical and biophysical research communications
- Publish Date: Jul 2006
- ISSN: 0006-291X
- Volume: 346
- Issue: 1
- Pages: 74-82
- Medium: Print
- Language: English
- Citation (JAMA): Mukai M, Endo H, Iwasaki T, et al. Rhoc is Essential for Tgf-beta1-induced Invasive Capacity of Rat Ascites Hepatoma Cells.. Biochem. Biophys. Res. Commun. Jul 2006;346:74-82
Abstract
Transforming growth factor-beta1 (TGF-beta1) is a multifunctional growth factor that plays a role in cell proliferation, differentiation, extracellular matrix production, apoptosis, and cell motility. We show here that TGF-beta1 increased the invasiveness of MM1 cells, which are a highly invasive clone of rat ascites hepatoma cells. Both mRNA and protein levels of RhoC but not RhoA in TGF-beta1-treated MM1 cells increased. In parallel with this increase in expression, RhoC activity was induced by TGF-beta1 treatment. When RhoC was overexpressed in MM1 cells, the invasive capacity increased. The RhoC-overexpressing cells formed more nodules than did mock cells when injected into rat peritoneum. Furthermore, when RhoC expression was reduced by transfection with shRNA/RhoC, the invasiveness of MM1 cells decreased with concomitant suppression of RhoC expression. Thus, the induced expression of RhoC by TGF-beta1 in MM1 cells plays a critical role in TGF-beta1-induced cell migration.
Mesh Headings (Keywords): Animals, Cells, Cultured, Gene Expression Profiling, Liver Neoplasms, Liver Neoplasms, Experimental, Neoplasm Invasiveness, Rats, Transforming Growth Factor beta, Transforming Growth Factor beta1, rho GTP-Binding Proteins, rhoA GTP-Binding Protein
Check for Full Text / PubMed Unique Identifier (PMID): 16750170
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