Reduction of the in Vitro Pro-inflammatory Response by Macrophages to Poly(3-hydroxybutyrate-co-3-hydroxyvalerate).
From: Institute for Molecular Bioscience and Co-operative Research Centre for Chronic Inflammatory Diseases, The University of Queensland, ST LUCIA 4072, Australia.
Biomaterials
- Publish Date: Sep 2006
- ISSN: 0142-9612
- Volume: 27
- Issue: 27
- Pages: 4715-25
- Medium: Print
- Language: English
- Citation (JAMA): Wu Andy C K, Grøndahl Lisbeth, Jack Kevin S, et al. Reduction of the in Vitro Pro-inflammatory Response by Macrophages to Poly(3-hydroxybutyrate-co-3-hydroxyvalerate).. Biomaterials Sep 2006;27:4715-25
Abstract
This study evaluates the pro-inflammatory response to the thermoplastic biopolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) through the analysis of cellular responses in vitro. The murine macrophage RAW264.7 cell line was cultured on solvent cast PHBV films, which was found to induce pro-inflammatory activity that required direct contact between the material and the macrophages. The identity of the pro-inflammatory stimulus was determined by culturing bone marrow-derived macrophages from bacterial lipopolysaccharide (LPS) hyporesponsive C3H/HeJ mice and CpG non-responsive TLR9-/- mice on PHBV. The lack of a pro-inflammatory response by the C3H/HeJ cells indicates that the pro-inflammatory agent present within PHBV is predominately LPS while the TLR9-/- macrophages confirmed that CpG-containing bacterial DNA is unlikely to contribute to the activity. A series of purification procedures was evaluated and one procedure was developed that utilized hydrogen peroxide treatment in solution. The optimized purification was found to substantially reduce the pro-inflammatory response to PHBV without adversely affecting either the molecular structure or molecular weight of the material thereby rendering it more amenable for use as a biomaterial in vivo.
Mesh Headings (Keywords): Animals, Biocompatible Materials, Cell Line, Histocompatibility Antigens Class II, Inflammation Mediators, Macrophage Activation, Macrophages, Mice, Mice, Inbred C3H, Mice, Inbred C57BL, Polyesters
Check for Full Text / PubMed Unique Identifier (PMID): 16750849
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