Level of Expression of Il-13r Alpha 2 Impacts Receptor Distribution and Il-13 Signaling.
From: Division of Allergy and Immunology, Department of Pediatrics, Cincinnati Children’ Hospital Medical Center, OH 45229, USA
Journal of immunology (Baltimore, Md. : 1950)
- Publish Date: Jun 2006
- ISSN: 0022-1767
- Volume: 176
- Issue: 12
- Pages: 7495-501
- Medium: Print
- Language: English
- Citation (JAMA): Daines Michael O, Tabata Yasuhiro, Walker Bradley A, et al. Level of Expression of Il-13r Alpha 2 Impacts Receptor Distribution and Il-13 Signaling.. J. Immunol. Jun 2006;176:7495-501
Abstract
IL-13, a critical cytokine for allergic inflammation, exerts its effects through a complex receptor system including IL-4Ralpha, IL-13Ralpha1, and IL-13Ralpha2. IL-4Ralpha and IL-13Ralpha1 form a heterodimeric signaling receptor for IL-13. In contrast, IL-13Ralpha2 binds IL-13 with high affinity but does not signal. IL-13Ralpha2 exists on the cell surface, intracellularly, and in soluble form, but no information is available regarding the relative distributions of IL-13Ralpha2 among these compartments, whether the compartments communicate, and how the relative expression levels impact IL-13 responses. Herein, we investigated the distribution of IL-13Ralpha2 in transfected and primary cells, and we evaluated how the total level of IL-13Ralpha2 expression impacted its distribution. Our results demonstrate that the distribution of IL-13Ralpha2 is independent of the overall level of expression. The majority of the IL-13Ralpha2 protein existed in intracellular pools. Surface IL-13Ralpha2 was continually released into the medium in a soluble form, yet surface expression remained constant supporting receptor trafficking to the cell surface. IL-13Ralpha2 inhibited IL-13 signaling proportionally to its level of expression, and this inhibition could be overcome with high concentrations of IL-13.
Mesh Headings (Keywords): Animals, Cell Membrane, Clone Cells, Dose-Response Relationship, Immunologic, Humans, Interleukin-13, Interleukin-13 Receptor alpha1 Subunit, Interleukin-4, Intracellular Fluid, Ligands, Mice, Mice, Inbred C3H, Protein Binding, Receptors, IgE, Receptors, Interleukin, Receptors, Interleukin-13, Signal Transduction, Solubility, Spleen, Transfection, U937 Cells
Check for Full Text / PubMed Unique Identifier (PMID): 16751396
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