Gas Chromatography-mass Spectrometry.
From: Department of Clinical Biochemistry, University College London Hospitals, UK.
Methods in molecular biology (Clifton, N.J.)
- Publish Date: 2006
- ISSN: 1064-3745
- Volume: 324
- Issue:
- Pages: 53-74
- Medium: Print
- Language: English
- Citation (JAMA): Honour John W, et al. Gas Chromatography-mass Spectrometry.. Methods Mol. Biol. 2006;324:53-74
Abstract
Gas chromatography-mass spectrometry (GC-MS) has been used in hormone assays particularly for steroids in biological fluids. The combination of GC with MS exploits the high-resolving power of gas chromatography to separate closely related molecules, and the ability of the MS to provide precise data for identification and quantification of the separated substances. GC-MS is a very powerful technique for analysis with specificity of hormones in biological fluids. The general principles of GC-MS are described in this chapter along with some examples that illustrate specific applications of hormone analysis.
Mesh Headings (Keywords): Adrenal Cortex Hormones, Animals, Gas Chromatography-Mass Spectrometry, Gonadal Steroid Hormones, Humans, Thyroid Hormones
Check for Full Text / PubMed Unique Identifier (PMID): 16761371
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