An Introduction to Pcr Techniques.
From: EurClinChem Department of Clinical Biochemistry, University College London Hospitals, London, UK.
Methods in molecular biology (Clifton, N.J.)
- Publish Date: 2006
- ISSN: 1064-3745
- Volume: 324
- Issue:
- Pages: 75-89
- Medium: Print
- Language: English
- Citation (JAMA): Rumsby Gill, et al. An Introduction to Pcr Techniques.. Methods Mol. Biol. 2006;324:75-89
Abstract
The polymerase chain reaction (PCR) is a method for the enzymatic amplification of DNA and has revolutionised our approach to both diagnostics and research, permitting the amplification of small amounts of genetic material, either derived from genomic (chromosomal DNA) or RNA via reverse transcription to cDNA. This chapter gives a primer of methods for the preparation of materials for PCR and some advice on the requirements for the PCR itself.
Mesh Headings (Keywords): Animals, Electrophoresis, Agar Gel, Humans, Polymerase Chain Reaction, RNA, Messenger
Check for Full Text / PubMed Unique Identifier (PMID): 16761372
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