Regulation of the Alpha-fetoprotein Promoter: Ku Binding and Dna Spatial Conformation.
From: Laboratoire de Biologie du Développement, Institut de Biologie et de Médecine Moléculaires, Université Libre de Bruxelles, 12, rue Professeurs-Jeener-et-amp-Brachet, 6041 Gosselies (Charleroi), Belgium.
Biochimie
- Publish Date: Oct 2006
- ISSN: 0300-9084
- Volume: 88
- Issue: 10
- Pages: 1409-17
- Medium: Print
- Language: English
- Citation (JAMA): Liénard P, De Mees C, Drèze P-L, et al. Regulation of the Alpha-fetoprotein Promoter: Ku Binding and Dna Spatial Conformation.. Biochimie Oct 2006;88:1409-17
Abstract
This work shows that the proximal promoter of the mouse Afp gene contains a Ku binding site and that Ku binding is associated with down-regulation of the transcriptional activity of the Afp promoter. The Ku binding site is located in a segment able to adopt a peculiar structured form, probably a hairpin structure. Interestingly, the structured form eliminates the binding sites of the positive transcription factor HNF1. Furthermore, a DNAse hypersensitive site is detected in footprinting experiments done with extracts of AFP non-expressing hepatoma cells. These observations suggest that the structured form is stabilised by Ku and is associated with extinction of the gene in AFP non-expressing hepatic cells.
Mesh Headings (Keywords): Animals, Antigens, Nuclear, Base Sequence, Binding Sites, Cell Line, Tumor, DNA, DNA-Binding Proteins, Gene Expression Regulation, Hepatocyte Nuclear Factor 1, Humans, Mice, Molecular Sequence Data, Nucleic Acid Conformation, Promoter Regions (Genetics), Rats, alpha-Fetoproteins
Check for Full Text / PubMed Unique Identifier (PMID): 16765502
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