E1a is the Component of the Mhc Class I Enhancer Complex That Mediates Hdac Chromatin Repression in Adenovirus-12 Tumorigenic Cells.
From: Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Levy Research Building, Room 221, 4010 Locust Street, Philadelphia, PA 19104, USA.
Virology
- Publish Date: Sep 2006
- ISSN: 0042-6822
- Volume: 352
- Issue: 2
- Pages: 338-44
- Medium: Print
- Language: English
- Citation (JAMA): Zhao Biwei, Ricciardi Robert P, et al. E1a is the Component of the Mhc Class I Enhancer Complex That Mediates Hdac Chromatin Repression in Adenovirus-12 Tumorigenic Cells.. Virology Sep 2006;352:338-44
Abstract
In adenovirus-12 tumorigenic cells, the viral E1A-12 protein mediates transcriptional down-regulation of the major histocompatibility complex (MHC) class I genes by targeting the class I enhancer. Here, we demonstrate by a combination of antisense and chromatin immunoprecipitation (ChIP) analysis that E1A-12 is a physical component of the class I enhancer repression complex, known to comprise COUP-TFII and histone deacetylase 1 (HDAC1). Significantly, E1A antisense was shown to co-eliminate E1A-12 as well as HDAC1 and HDAC8, but not HDAC3, from the enhancer repression complex. Consistent with elimination of HDAC1 and HDAC8, E1A antisense also resulted in a dramatic increase in histone acetylation, a hallmark of transcriptionally active chromatin. Importantly, MHC class I antigen expression was restored on the surface of E1A antisense-transfected cells. These results demonstrate that E1A-12 is associated with the MHC class I complex and apparently mediates class I transcriptional down-regulation by enacting chromatin repression through HDAC1 and HDAC8.
Mesh Headings (Keywords): Adenoviridae, Adenovirus E1A Proteins, Animals, Base Sequence, COS Cells, Cell Line, Tumor, Cercopithecus aethiops, Chromatin, Enhancer Elements (Genetics), Genes, MHC Class I, Histocompatibility Antigens Class I, Histone Deacetylases, Mice, Oligodeoxyribonucleotides, Antisense
Check for Full Text / PubMed Unique Identifier (PMID): 16780916
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