Distribution of Ephb Receptors and Ephrin-b1 in the Developing Vertebrate Spinal Cord.
From: Department of Pathology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
The Journal of comparative neurology
- Publish Date: Aug 2006
- ISSN: 0021-9967
- Volume: 497
- Issue: 5
- Pages: 734-50
- Medium: Print
- Language: English
- Citation (JAMA): Jevince Angela R, Kadison Stephanie R, Pittman Andrew J, et al. Distribution of Ephb Receptors and Ephrin-b1 in the Developing Vertebrate Spinal Cord.. J. Comp. Neurol. Aug 2006;497:734-50
Abstract
Contact-dependent interactions between EphB receptors and ephrin-B ligands mediate a variety of cell-cell communication events in the developing and mature central nervous system (CNS). These predominantly repulsive interactions occur at the interface between what are considered to be mutually exclusive EphB and ephrin-B expression domains. We previously used receptor and ligand affinity probes to show that ephrin-B ligands are expressed in the floor plate and within a dorsal region of the embryonic mouse spinal cord, while EphB receptors are present on decussated segments of commissural axons that navigate between these ephrin-B domains. Here we present the generation and characterization of two new monoclonal antibodies, mAb EfB1-3, which recognizes EphB1, EphB2, and EphB3, and mAb efrnB1, which is specific for ephrin-B1. We use these reagents and polyclonal antibodies specific for EphB1, EphB2, EphB3, or ephrin-B1 to describe the spatiotemporal expression patterns of EphB receptors and ephrin-B1 in the vertebrate spinal cord. Consistent with affinity probe binding, we show that EphB1, EphB2, and EphB3 are each preferentially expressed on decussated segments of commissural axons in vivo and in vitro, and that ephrin-B1 is expressed in a dorsal domain of the spinal cord that includes the roof plate. In contrast to affinity probe binding profiles, we show here that EphB1, EphB2, and EphB3 are present on the ventral commissure, and that EphB1 and EphB3 are expressed on axons that compose the dorsal funiculus. In addition, we unexpectedly find that mesenchymal cells, which surround the spinal cord and dorsal root ganglion, express ephrin-B1.
Mesh Headings (Keywords): Animals, Axons, Chick Embryo, Cricetinae, Ephrin-B1, Ephrin-B3, Female, Immunohistochemistry, Mice, Mice, Inbred BALB C, Rats, Rats, Sprague-Dawley, Receptor, EphB2, Receptors, Eph Family, Spinal Cord, Tissue Distribution, Zebrafish
Check for Full Text / PubMed Unique Identifier (PMID): 16786562
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.