Purification and in Vitro Characterization of Adult Canine Olfactory Ensheathing Cells.
From: Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, Germany.
Cell and tissue research
- Publish Date: Dec 2006
- ISSN: 0302-766X
- Volume: 326
- Issue: 3
- Pages: 687-96
- Medium: Print
- Language: English
- Citation (JAMA): Krudewig Christiane, Deschl Ulrich, Wewetzer Konstantin, et al. Purification and in Vitro Characterization of Adult Canine Olfactory Ensheathing Cells.. Cell Tissue Res. Dec 2006;326:687-96
Abstract
Olfactory ensheathing cells (OECs) are known to promote neural repair under experimental conditions. The experimental focus has so far been almost entirely on rodent OECs (rOECs), and hence whether human OECs (humOECs) display similar properties is unclear. Studies on larger mammals as an “intermediate” model may be helpful for translating the experimental evidence gathered so far into novel therapeutic strategies. In the present study, we purified adult canine OECs (caOECs) from the olfactory bulb and analyzed their in vitro properties with respect to antigen expression, proliferation, and differentiation. Secondary caOECs shared the expression of marker molecules and the reactivity toward growth factors, with rOECs and humOECs. CaOECs were positively immunostained for the low affinity neurotrophin receptor p75, GFAP, and O4 and proliferated in response to fibroblast growth factor-2 and heregulin-1beta. No decline in proliferation was noted at higher passages (>8). The effects of forskolin, which neither increased proliferation nor stimulated the expression of O4, were clearly different from those on rOECs. Moreover, caOECs displayed their typical spindle-shaped morphology only upon growth factor/forskolin addition, whereas mitotically quiescent caOECs had a flattened morphology. Thus, caOECs can readily be purified from adult canine olfactory bulb and expanded by using established OEC mitogens. The behavior of caOECs toward forskolin suggests that caOECs and humOECs share a number of properties amd implies the presence of common intracellular signalling pathways. CaOECs therefore represent a suitable model system relevant for humOECs in neural repair studies.
Mesh Headings (Keywords): Animals, Cell Differentiation, Cell Proliferation, Cells, Cultured, Culture Media, Serum-Free, Dogs, Dose-Response Relationship, Drug, Fibroblast Growth Factor 2, Fluorescent Antibody Technique, Indirect, Forskolin, Glial Fibrillary Acidic Protein, Immunohistochemistry, Neuregulin-1, Neuroglia, Olfactory Bulb, Receptor, Nerve Growth Factor, Time Factors
Check for Full Text / PubMed Unique Identifier (PMID): 16788833
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