Cell-cell Interactions on Solid Matrices.
From: Center for Experimental Therapeutics and Reperfusion Injury, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA.
Methods in molecular biology (Clifton, N.J.)
- Publish Date: 2006
- ISSN: 1064-3745
- Volume: 341
- Issue:
- Pages: 51-9
- Medium: Print
- Language: English
- Citation (JAMA): Louis Nancy A, Daniels Dionne, Colgan Sean P, et al. Cell-cell Interactions on Solid Matrices.. Methods Mol. Biol. 2006;341:51-9
Abstract
Models to study molecular, biochemical, and functional responses in vitro generally incorporate an individual cell type or group of cells organized in a random fashion. Normal physiological responses in vivo require that individual cell types be oriented in an organized fashion with three-dimensional architecture and appropriately positioned cellular interfaces. Much recent progress has been made in the development and implementation of models to study cell-cell contact using substrate grown cells. Here, we summarize the use of membrane permeable supports to study functional responses in appropriately positioned cell types. These models incorporate two or more different cells cultured in physiologically positioned locales on solid substrates. Models incorporating nonadherent cells (e.g., leukocytes) in co-culture with such models also are discussed. Such models have been used extensively to discovery both cell-bound as well as soluble mediators of physiological and pathophysiological processes.
Mesh Headings (Keywords): Animals, Cell Adhesion, Cell Communication, Cell Membrane Permeability, Coculture Techniques, Endothelial Cells, Extracellular Matrix, Humans, Leukocytes, Models, Biological
Check for Full Text / PubMed Unique Identifier (PMID): 16799188
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.