Deletion of Endothelial Cell Endothelin B Receptors Does Not Affect Blood Pressure or Sensitivity to Salt.
From: Centre for Cardiovascular Science, University of Edinburgh, Queen’s Medical Research Institute, Little France Crescent, Edinburgh, EH16 4TJ United Kingdom. Alan.Bagnall@ed.ac.uk
Hypertension
- Publish Date: Aug 2006
- ISSN: 1524-4563
- Volume: 48
- Issue: 2
- Pages: 286-93
- Medium: Internet
- Language: English
- Citation (JAMA): Bagnall Alan J, Kelland Nicholas F, Gulliver-Sloan Fiona, et al. Deletion of Endothelial Cell Endothelin B Receptors Does Not Affect Blood Pressure or Sensitivity to Salt.. Hypertension Aug 2006;48:286-93
Abstract
Endothelin B receptors in different tissues regulate diverse physiological responses including vasoconstriction, vasodilatation, clearance of endothelin-1, and renal tubular sodium reabsorption. To examine the role of endothelial cell endothelin B receptors in these processes, we generated endothelial cell-specific endothelin B receptor knockout mice using a Cre-loxP approach. We have demonstrated loss of endothelial cell endothelin B receptor expression and function and preservation of nonendothelial endothelin B receptor-mediated responses through binding and functional assays. Ablation of endothelin B receptors exclusively from endothelial cells produces endothelial dysfunction in the absence of hypertension, with evidence of decreased endogenous release of NO and increased plasma endothelin-1. In contrast to models of total endothelin B receptor ablation, the blood pressure response to a high-salt diet is unchanged in endothelial cell-specific endothelin B receptor knockouts compared with control floxed mice. These findings suggest that the endothelial cell endothelin B receptor mediates a tonic vasodilator effect and that nonendothelial cell endothelin B receptors are important for the regulation of blood pressure.
Mesh Headings (Keywords): Age Factors, Animals, Aorta, Binding, Competitive, Blood Pressure, Endothelial Cells, Endothelin-1, Gene Targeting, Heterozygote, Homozygote, Hypertension, Male, Mice, Mice, Knockout, Nitric Oxide, Organ Culture Techniques, Receptor, Endothelin B, Sodium Chloride, Dietary, Vasodilation
Check for Full Text / PubMed Unique Identifier (PMID): 16801484
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