Znf325, a Novel Human Zinc Finger Protein with a Rbak-like Rb-binding Domain, Inhibits Ap-1- and Sre-mediated Transcriptional Activity.
From: The Center for Heart Development, Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, Changsha, 410081 Hunan, People’s Republic of China.
Biochemical and biophysical research communications
- Publish Date: Aug 2006
- ISSN: 0006-291X
- Volume: 346
- Issue: 4
- Pages: 1191-9
- Medium: Print
- Language: English
- Citation (JAMA): Zhao Yulian, Zhou Liang, Liu Bisheng, et al. Znf325, a Novel Human Zinc Finger Protein with a Rbak-like Rb-binding Domain, Inhibits Ap-1- and Sre-mediated Transcriptional Activity.. Biochem. Biophys. Res. Commun. Aug 2006;346:1191-9
Abstract
Mitogen-activated protein kinase (MAPK) signal transduction pathways are among the most widespread mechanisms of eukaryotic cell regulation. The zinc-finger-containing transcription factors have been previously revealed to be involved in the regulation of the MAPK signaling pathways. Here, we have identified a novel human zinc-finger transcriptional repressor, ZNF325, that contains a RBaK-like RB-binding domain and 15 tandem repeated C2H2 type zinc fingers. Northern blot analysis indicates that a 2.7 kb transcript specific for ZNF325 is widely expressed in all tissues examined at adult stage and in most of the embryonic tissues. Overexpression of ZNF325 in COS-7 cells inhibits the transcriptional activities of AP-1 and SRE. The deletion and RNAi analysis indicate that the C2H2 zinc finger motifs represent the basal transcriptional repressive activity. These results indicate that the ZNF325 protein may act as a novel transcription repressor in MAPK signaling pathway to mediate cellular functions.
Mesh Headings (Keywords): Amino Acid Sequence, Base Sequence, Binding Sites, DNA, Complementary, DNA-Binding Proteins, Humans, Kruppel-Like Transcription Factors, Mitogen-Activated Protein Kinases, Molecular Sequence Data, Retinoblastoma Protein, Serum Response Element, Signal Transduction, Transcription Factor AP-1, Transcription Factors, Transcription, Genetic, Zinc Fingers
Check for Full Text / PubMed Unique Identifier (PMID): 16806083
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.