Crystal Structure of the Bacterial Ribosomal Decoding Site Complexed with Amikacin Containing the Gamma-amino-alpha-hydroxybutyryl (Haba) Group.
From: Institut de Biologie Moléculaire et Cellulaire, UPR9002 CNRS, Université Louis-Pasteur, 15, rue René-Descartes, 67084 Strasbourg, France.
Biochimie
- Publish Date: Aug 2006
- ISSN: 0300-9084
- Volume: 88
- Issue: 8
- Pages: 1027-31
- Medium: Print
- Language: English
- Citation (JAMA): Kondo Jiro, François Boris, Russell Rupert J M, et al. Crystal Structure of the Bacterial Ribosomal Decoding Site Complexed with Amikacin Containing the Gamma-amino-alpha-hydroxybutyryl (Haba) Group.. Biochimie Aug 2006;88:1027-31
Abstract
Amikacin is the 4,6-linked aminoglycoside modified at position N1 of the 2-deoxystreptamine ring (ring II) by the L-haba group. In the present study, the crystal structure of a complex between oligonucleotide containing the bacterial ribosomal A site and amikacin has been solved at 2.7 A resolution. Amikacin specifically binds to the A site in practically the same way as its parent compound kanamycin. In addition, the L-haba group interacts with the upper side of the A site through two direct contacts, O2*…H-N4(C1496) and N4*-H…O6(G1497). The present crystal structure shows how the introduction of the L-haba group on ring II of aminoglycoside is an effective mutation for obtaining a higher affinity to the bacterial A site.
Mesh Headings (Keywords): Amikacin, Binding Sites, Crystallography, X-Ray, Models, Molecular, Nucleic Acid Conformation, Oligonucleotides, RNA, Ribosomal, 16S, Ribosomes
Check for Full Text / PubMed Unique Identifier (PMID): 16806634
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