New Restriction Fragment Length Polymorphisms in the Cytochrome Oxidase I Gene Facilitate Host Strain Identification of Fall Armyworm (Lepidoptera: Noctuidae) Populations in the Southeastern United States.
From: Center for Medical, Agricultural and Veterinary Entomology, USDA-ARS, Gainesville, FL 32608, USA.
Journal of economic entomology
- Publish Date: Jun 2006
- ISSN: 0022-0493
- Volume: 99
- Issue: 3
- Pages: 671-7
- Medium: Print
- Language: English
- Citation (JAMA): Nagoshi Rod N, Meagher Robert L, Adamczyk John J, et al. New Restriction Fragment Length Polymorphisms in the Cytochrome Oxidase I Gene Facilitate Host Strain Identification of Fall Armyworm (Lepidoptera: Noctuidae) Populations in the Southeastern United States.. J. Econ. Entomol. Jun 2006;99:671-7
Abstract
Several restriction sites in the cytochrome oxidase I gene of fall armyworm, Spodoptera frugiperda (J.E. Smith), were identified by sequence analysis as potentially being specific to one of the two host strains. Strain specificity was demonstrated for populations in Florida, Texas, Mississippi, Georgia, and North Carolina, with an AciI and SacI site specific to the rice (Oryjza spp.)-strain and a BsmI and HinfI site joining an already characterized MspI site as diagnostic of the corn (Zea mays L.)-strain. All four of these sites can be detected by digestion of a single 568-bp polymerase chain reaction-amplified fragment, but the use of two enzymes in separate digests was found to provide accurate and rapid determination of strain identity. The effectiveness of this method was demonstrated by the analysis of almost 200 adult and larval specimens from the Mississippi delta region. The results indicated that the corn-strain is likely to be the primary strain infesting cotton (Gossypium spp.) and that an unexpected outbreak of fall armyworm on the ornamental tree Paulownia tomentosa (Thunb.) Sieb. & Zucc. ex Steud. was due almost entirely to the rice-strain.
Mesh Headings (Keywords): Animals, Electron Transport Complex IV, Life Cycle Stages, Male, Plants, Polymorphism, Restriction Fragment Length, Southeastern United States, Spodoptera
Check for Full Text / PubMed Unique Identifier (PMID): 16813297
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