Conserved Sol-1 Proteins Regulate Ionotropic Glutamate Receptor Desensitization.
From: Department of Biology, University of Utah, Salt Lake City, UT 84112-0840, USA.
Proceedings of the National Academy of Sciences of the United States of America
- Publish Date: Jul 2006
- ISSN: 0027-8424
- Volume: 103
- Issue: 28
- Pages: 10787-92
- Medium: Print
- Language: English
- Citation (JAMA): Walker Craig S, Francis Michael M, Brockie Penelope J, et al. Conserved Sol-1 Proteins Regulate Ionotropic Glutamate Receptor Desensitization.. Proc. Natl. Acad. Sci. U.S.A. Jul 2006;103:10787-92
Abstract
The neurotransmitter glutamate mediates excitatory synaptic transmission by activating ionotropic glutamate receptors (iGluRs). In Caenorhabditis elegans, the GLR-1 receptor subunit is required for glutamate-gated current in a subset of interneurons that control avoidance behaviors. Current mediated by GLR-1-containing iGluRs depends on SOL-1, a transmembrane CUB-domain protein that immunoprecipitates with GLR-1. We have found that reconstitution of glutamate-gated current in heterologous cells depends on three proteins, STG-1 (a C. elegans stargazin-like protein), SOL-1, and GLR-1. Here, we use genetic and pharmacological perturbations along with rapid perfusion electrophysiological techniques to demonstrate that SOL-1 functions to slow the rate and limit the extent of receptor desensitization as well as to enhance the recovery from desensitization. We have also identified a SOL-1 homologue from Drosophila and show that Dro SOL1 has a conserved function in promoting C. elegans glutamate-gated currents. SOL-1 homologues may play critical roles in regulating glutamatergic neurotransmission in more complex nervous systems.
Mesh Headings (Keywords): Amino Acid Sequence, Animals, Animals, Genetically Modified, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Cells, Cultured, Concanavalin A, Conserved Sequence, Drosophila Proteins, Membrane Proteins, Molecular Sequence Data, Receptors, AMPA
Check for Full Text / PubMed Unique Identifier (PMID): 16818875
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