Differential Effect of Orexins (Hypocretins) on Serotonin Release in the Dorsal and Median Raphe Nuclei of Freely Behaving Rats.
From: Boston VA Healthcare/Harvard Medical School, Department of Psychiatry, Laboratory of Neuroscience, 940 Belmont Street, Brockton, MA 02301, USA. rtao@fau.edu
Neuroscience
- Publish Date: Sep 2006
- ISSN: 0306-4522
- Volume: 141
- Issue: 3
- Pages: 1101-5
- Medium: Print
- Language: English
- Citation (JAMA): Tao R, Ma Z, McKenna J T, et al. Differential Effect of Orexins (Hypocretins) on Serotonin Release in the Dorsal and Median Raphe Nuclei of Freely Behaving Rats.. Neuroscience Sep 2006;141:1101-5
Abstract
Orexin (hypocretin)-containing neurons in the perifornical hypothalamus project to widespread regions of the brain, including the dorsal and median raphe nuclei [Peyron C, Tighe DK, van den Pol AN, de Lecea L, Heller HC, Sutcliffe JG, Kilduff TS (1998) Neurons containing hypocretin (orexin) project to multiple neuronal systems. J Neurosci 18:9996-10015; Wang QP, Koyama Y, Guan JL, Takahashi K, Kayama Y, Shioda S (2005) The orexinergic synaptic innervation of serotonin- and orexin 1-receptor-containing neurons in the dorsal raphe nucleus. Regul Pept 126:35-42]. Orexin-A or orexin-B was infused by reverse microdialysis into the dorsal raphe nucleus or median raphe nucleus of freely behaving rats, and extracellular serotonin was simultaneously collected by microdialysis and analyzed by high-performance liquid chromatography. We have found that orexin-A produced a dose-dependent increase of serotonin in the dorsal raphe nucleus, but not in the median raphe nucleus. However, orexin-B elicited a small but significant effect in both the dorsal raphe nucleus and median raphe nucleus. Orexins may have regionally selective effects on serotonin release in the CNS, implying a unique interaction between orexins and serotonin in the regulation of activities including sleep-wakefulness.
Mesh Headings (Keywords): Analysis of Variance, Animals, Dose-Response Relationship, Drug, Intracellular Signaling Peptides and Proteins, Male, Microdialysis, Neuropeptides, Raphe Nuclei, Rats, Rats, Sprague-Dawley, Serotonin
Check for Full Text / PubMed Unique Identifier (PMID): 16820265
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