Myogenic Potential of Adipose-tissue-derived Cells.
From: Laboratorio di Biologia Vascolare e Terapia Genica, Centro Cardiologico Fondazione Monzino, Via Parea 4, 20138 Milan, Italy.
Journal of cell science
- Publish Date: Jul 2006
- ISSN: 0021-9533
- Volume: 119
- Issue: Pt 14
- Pages: 2945-52
- Medium: Print
- Language: English
- Citation (JAMA): Di Rocco Giuliana, Iachininoto Maria Grazia, Tritarelli Alessandra, et al. Myogenic Potential of Adipose-tissue-derived Cells.. J. Cell. Sci. Jul 2006;119:2945-52
Abstract
Adipose-tissue-derived mesenchymal stem cells can be directed towards a myogenic phenotype in vitro by the addition of specific inductive media. However, the ability of these or other adipose-tissue-associated cells to respond to ;natural’ myogenic cues such as a myogenic environment has never been investigated in detail. Here, we provide evidence that a restricted subpopulation of freshly harvested adipose-tissue-derived cells possesses an intrinsic myogenic potential and can spontaneously differentiate into skeletal muscle. Conversion of adipose-tissue-derived cells to a myogenic phenotype is enhanced by co-culture with primary myoblasts in the absence of cell contact and is maximal when the two cell types are co-cultured in the same plate. Conversely, in vitro expanded adipose-tissue-derived mesenchymal stem cells require direct contact with muscle cells to generate skeletal myotubes. Finally, we show that uncultured adipose-tissue-associated cells have a high regenerative capacity in vivo since they can be incorporated into muscle fibers following ischemia and can restore significantly dystrophin expression in mdx mice.
Mesh Headings (Keywords): Adipose Tissue, Animals, Cell Differentiation, Cell Transplantation, Cells, Cultured, Coculture Techniques, Dystrophin, Mesenchymal Stem Cells, Mice, Mice, Inbred mdx, Muscle Fibers, Muscle, Skeletal, Myoblasts, Skeletal, Myocardial Ischemia, Regeneration, Stromal Cells
Check for Full Text / PubMed Unique Identifier (PMID): 16825428
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