Transient Suppression of Ppargamma Directed Es Cells into an Osteoblastic Lineage.
From: Research Center for Animal Life Science, Shiga University of Medical Science, Tsukinowa-cho, Seta, Ohtsu 520-2192, Japan.
FEBS letters
- Publish Date: Jul 2006
- ISSN: 0014-5793
- Volume: 580
- Issue: 17
- Pages: 4121-5
- Medium: Print
- Language: English
- Citation (JAMA): Yamashita Akihiro, Takada Tatsuyuki, Nemoto Ken-ichi, et al. Transient Suppression of Ppargamma Directed Es Cells into an Osteoblastic Lineage.. FEBS Lett. Jul 2006;580:4121-5
Abstract
Osteoblasts and adipocytes are believed to share a common progenitor. Peroxisome proliferator-activated receptor gamma (PPARgamma) plays a key role in the switching of these two cell lineages. Here, we demonstrated the differentiation of ES cells into an osteoblastic lineage using siRNA against PPARgamma without the addition of any osteogenic factors. We found that PPARgamma-siRNA downregulated the expression of aP2 mRNA and lipid accumulation, whereas it upregulated the expression of osteocalcin and calcium deposition. These results suggested that ES cells were directed into an osteoblastic lineage. Therefore, transient suppression using PPARgamma-siRNA may be a novel tool to induce differentiation of ES cells into osteoblasts.
Mesh Headings (Keywords): Adipocytes, Animals, Calcification, Physiologic, Calcium, Cell Differentiation, Cell Line, Cell Lineage, Embryo, Mammalian, Mice, Osteoblasts, Osteopontin, PPAR gamma, RNA, Messenger, RNA, Small Interfering, Sialoglycoproteins, Stem Cells, Transcription Factors, Up-Regulation
Check for Full Text / PubMed Unique Identifier (PMID): 16828750
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