Amplification of Chromosome 21q22.3 Harboring Trefoil Factor Family Genes in Liver Fluke Related Cholangiocarcinoma is Associated with Poor Prognosis.
From: Department of Clinical Chemistry, Center for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
World journal of gastroenterology : WJG
- Publish Date: Jul 2006
- ISSN: 1007-9327
- Volume: 12
- Issue: 26
- Pages: 4143-8
- Medium: Print
- Language: English
- Citation (JAMA): Muenphon Kanuengnuch, Limpaiboon Temduang, Jearanaikoon Patcharee, et al. Amplification of Chromosome 21q22.3 Harboring Trefoil Factor Family Genes in Liver Fluke Related Cholangiocarcinoma is Associated with Poor Prognosis.. World J. Gastroenterol. Jul 2006;12:4143-8
Abstract
AIM: To determine allelic imbalance on chromosomal region 21q22-qter including trefoil factor family genes (TFF) in cholangiocarcinoma (CCA) patients and analyze the correlation between allelic imbalances and clinicopathological parameters. METHODS: Quantitative PCR amplification was performed on four microsatellite markers and trefoil factor family genes (TFF1, TFF2, and TFF3) using a standard curve and SYBR Green I dye method. The relative copy number was determined by DNA copy number of tested locus to reference locus. The relative copy number was interpreted as deletion or amplification by comparison with normal reference range. Associations between allelic imbalance and clinicopathological parameters of CCA patients were evaluated by chi(2)-tests. Kaplan-Meier method was used to analyze survival. RESULTS: The frequencies of amplification at D21S1890, D21S1893, and TFF3 were 32.5%, 30.0%, and 28.7%, respectively. Patients who had amplification at regions covering D21S1893, D21S1890, and TFF showed poor prognosis, whereas patients who had deletion showed favorable prognosis (mean: 51.7 wk vs 124.82 wk, P = 0.012). Multivariate Cox regression analysis revealed that amplification of D21S1893, D21S1890 and TFF, blood vessel invasion, and staging were associated with poor prognosis. CONCLUSION: D21S1893-D21S1890 region may harbor candidate genes especially TFF and serine protease family, which might be involved in tumor invasion and metastasis contributing to poor survival. The amplification in this region may be used as a prognostic marker in the treatment of CCA patients.
Mesh Headings (Keywords): Alleles, Animals, Bile Duct Neoplasms, Bile Ducts, Intrahepatic, Cholangiocarcinoma, Chromosome Mapping, Chromosomes, Human, Pair 21, DNA, Neoplasm, Fasciola hepatica, Fascioliasis, Female, Gene Amplification, Gene Frequency, Genes, Neoplasm, Humans, Male, Middle Aged, Multivariate Analysis, Neoplasm Invasiveness, Neoplasm Metastasis, Peptides, Prognosis, Tumor Markers, Biological
Check for Full Text / PubMed Unique Identifier (PMID): 16830362
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