Tissue-specific Chromatin Modifications at a Multigene Locus Generate Asymmetric Transcriptional Interactions.
From: Department of Genetics, University of Pennsylvania School of Medicine, 415 Curie Blvd., 428 Clinical Research Building, Philadelphia, PA 19104, USA.
Molecular and cellular biology
- Publish Date: Aug 2006
- ISSN: 0270-7306
- Volume: 26
- Issue: 15
- Pages: 5569-79
- Medium: Print
- Language: English
- Citation (JAMA): Yoo Eung Jae, Cajiao Isabela, Kim Jeong-Seon, et al. Tissue-specific Chromatin Modifications at a Multigene Locus Generate Asymmetric Transcriptional Interactions.. Mol. Cell. Biol. Aug 2006;26:5569-79
Abstract
Random assortment within mammalian genomes juxtaposes genes with distinct expression profiles. This organization, along with the prevalence of long-range regulatory controls, generates a potential for aberrant transcriptional interactions. The human CD79b/GH locus contains six tightly linked genes with three mutually exclusive tissue specificities and interdigitated control elements. One consequence of this compact organization is that the pituitary cell-specific transcriptional events that activate hGH-N also trigger ectopic activation of CD79b. However, the B-cell-specific events that activate CD79b do not trigger reciprocal activation of hGH-N. Here we utilized DNase I hypersensitive site mapping, chromatin immunoprecipitation, and transgenic models to explore the basis for this asymmetric relationship. The results reveal tissue-specific patterns of chromatin structures and transcriptional controls at the CD79b/GH locus in B cells distinct from those in the pituitary gland and placenta. These three unique transcriptional environments suggest a set of corresponding gene expression pathways and transcriptional interactions that are likely to be found juxtaposed at multiple sites within the eukaryotic genome.
Mesh Headings (Keywords): Animals, Antigens, CD79, B-Lymphocytes, Cell Line, Chromatin, Deoxyribonuclease I, Gene Expression Regulation, Histones, Human Growth Hormone, Humans, Locus Control Region, Mice, Mice, Transgenic, Transcription, Genetic
Check for Full Text / PubMed Unique Identifier (PMID): 16847312
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.