Wnt2b/Beta-catenin-mediated Canonical Wnt Signaling Determines the Peripheral Fates of the Chick Eye.
From: Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.
Development (Cambridge, England)
- Publish Date: Aug 2006
- ISSN: 0950-1991
- Volume: 133
- Issue: 16
- Pages: 3167-77
- Medium: Print
- Language: English
- Citation (JAMA): Cho Seo-Hee, Cepko Constance L, et al. Wnt2b/Beta-catenin-mediated Canonical Wnt Signaling Determines the Peripheral Fates of the Chick Eye.. Development Aug 2006;133:3167-77
Abstract
Wnt signaling orchestrates multiple aspects of central nervous system development, including cell proliferation and cell fate choices. In this study, we used gene transfer to activate or inhibit canonical Wnt signaling in vivo in the developing eye. We found that the expression of Wnt2b or constitutively active (CA) beta-catenin inhibited retinal progenitor gene (RPG) expression and the differentiation of retinal neurons. In addition, Wnt signal activation in the central retina was sufficient to induce the expression of markers of the ciliary body and iris, two tissues derived from the peripheral optic cup (OC). The expression of a dominant-negative (DN) allele of Lef1, or of a Lef1-engrailed fusion protein, led to the inhibition of expression of peripheral genes and iris hypoplasia, suggesting that canonical Wnt signaling is required for peripheral eye development. We propose that canonical Wnt signaling in the developing optic vesicle (OV) and OC plays a crucial role in determining the identity of the ciliary body and iris. Because wingless (wg) plays a similar role in the induction of peripheral eye tissues of Drosophila, these findings indicate a possible conservation of the process that patterns the photoreceptive and support structures of the eye.
Mesh Headings (Keywords): Animals, Biological Markers, Cell Differentiation, Cell Proliferation, Chick Embryo, Ciliary Body, Eye, Gene Expression Regulation, Developmental, Iris, Neurons, Retina, Wnt2 Protein, beta Catenin
Check for Full Text / PubMed Unique Identifier (PMID): 16854977
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.