Response to Dynamic Strain in Human Periosteal Cells Grown in Vitro
From: Department of Orthopaedics, Navy General Hospital, Beijing 100037, China. zcmail2002@yahoo.com.cn
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
- Publish Date: Jun 2006
- ISSN: 1001-5515
- Volume: 23
- Issue: 3
- Pages: 546-50
- Medium: Print
- Language:
- Citation (JAMA): Zhang Chao, Liang Guosui, Zhang Yingkai, et al. Response to Dynamic Strain in Human Periosteal Cells Grown in Vitro. Jun 2006;23:546-50
Abstract
The objective of this program is to investigate the biological effect of dynamic strain on human periosteal cells in vitro. Using a well-established model, the Flexercell unit, we placed mechanical stress (50,000 microstrain, 1 Hz and sine wave) on human periosteal cells grown in collagen coated flexible membrane. The time points of proliferative and differentiative properties were assessed by means of cell counting, thymidine incorporation, synthesis of alkaline phosphatase and osteocalcin, and long term of mechanical load induced calcium nodules formation was also demonstrated. The results showed that the application of highly controlled strains exerted a significant effect on human periosteal cells by up regulation of osteogenic properties rather than exercised an influence on proliferation. The results suggested that the promoting effects of dynamic strain on human periosteal cells probably contribute to the biological function of mechanical loading bearing.
Mesh Headings (Keywords): Biomechanics, Cell Proliferation, Cells, Cultured, Humans, Periosteum, Stress, Mechanical, Ulna, Weight-Bearing
Check for Full Text / PubMed Unique Identifier (PMID): 16856386
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