Influenza A Virus Infection of Primary Differentiated Airway Epithelial Cell Cultures Derived from Syrian Golden Hamsters.
From: Department of Molecular Microbiology, Washington University in St. Louis School of Medicine, 660 S. Euclid Avenue, Campus Box 8230, St. Louis, MO 63110, USA.
Virology
- Publish Date: Oct 2006
- ISSN: 0042-6822
- Volume: 354
- Issue: 1
- Pages: 80-90
- Medium: Print
- Language: English
- Citation (JAMA): Newby Celeste M, Rowe Regina K, Pekosz Andrew, et al. Influenza A Virus Infection of Primary Differentiated Airway Epithelial Cell Cultures Derived from Syrian Golden Hamsters.. Virology Oct 2006;354:80-90
Abstract
The ability of several different influenza A virus strains to infect and replicate in primary, differentiated airway epithelial cell cultures from Syrian golden hamsters was investigated. All virus strains tested replicated equivalently in the cultures and displayed a preference for infecting nonciliated cells. This tropism correlated with the expression of both alpha2,3- and alpha2,6-linked sialic acid on the nonciliated cells. In contrast, the ciliated cells did not have detectable alpha2,6-linked sialic acid and expressed only low amounts of alpha2,3-linked sialic acid. In contrast to clinical isolates, laboratory strains of influenza A virus infected a limited number of ciliated cells at late times post-infection. The presence of alpha2,3- and alpha2,6-linked sialic acid residues on the same cell type suggests that Syrian golden hamsters and differentiated airway epithelial cell cultures derived from hamsters may provide a system for studying the reassortment of influenza A virus strains which utilize different forms of sialic acid as a primary virus receptor.
Mesh Headings (Keywords): Animals, Cells, Cultured, Cilia, Cricetinae, Epithelial Cells, Influenza A virus, Mesocricetus, N-Acetylneuraminic Acid, Plaque Assay, Receptors, Virus, Respiratory Mucosa, Virus Replication
Check for Full Text / PubMed Unique Identifier (PMID): 16876846
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