Spectroscopic Translation of Cell-material Interactions.
From: Northwestern University, Biomedical Engineering Department, 2145 Sheridan Rd., Room E310, Evanston, IL 60208, USA.
Biomaterials
- Publish Date: Jan 2007
- ISSN: 0142-9612
- Volume: 28
- Issue: 2
- Pages: 162-74
- Medium: Print
- Language: English
- Citation (JAMA): Allen Josephine, Liu Yang, Kim Young L, et al. Spectroscopic Translation of Cell-material Interactions.. Biomaterials Jan 2007;28:162-74
Abstract
The characterization of cellular interactions with a biomaterial surface is important to the development of novel biomaterials. Traditional methods used to characterize processes such as cellular adhesion and differentiation on biomaterials can be time consuming, and destructive, and are not amenable to quantitative assessment in situ. As the development of novel biomaterials shifts towards small-scale, combinatorial, and high throughput approaches, new techniques will be required to rapidly screen and characterize cell/biomaterial interactions. Towards this goal, we assessed the feasibility of using 4-dimensional elastic light-scattering fingerprinting (4D-ELF) to describe the differentiation of human aortic smooth muscle cells (HASMCs), as well as the adhesion, and apoptotic processes of human aortic endothelial cells (HAECs), in a quantitative and non-perturbing manner. HASMC and HAEC were cultured under conditions to induce cell differentiation, attachment, and apoptosis which were evaluated via immunohistochemistry, microscopy, biochemistry, and 4D-ELF. The results show that 4D-ELF detected changes in the size distributions of subcellular organelles and structures that were associated with these specific cellular processes. 4D-ELF is a novel way to assess cell phenotype, strength of adhesion, and the onset of apoptosis on a biomaterial surface and could potentially be used as a rapid and quantitative screening tool to provide a more in-depth understanding of cell/biomaterial interactions.
Mesh Headings (Keywords): Biocompatible Materials, Cell Adhesion, Cell Adhesion Molecules, Cells, Cultured, Cytoskeleton, Humans, Microscopy, Electron, Transmission, Muscle, Smooth, Vascular, Scattering, Radiation, Spectrum Analysis
Check for Full Text / PubMed Unique Identifier (PMID): 16884769
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.