Quantitative Profiling of Protein-dna Binding on Microarrays.
From: Wellcome Trust Center for Human Genetics, University of Oxford, Oxford, UK.
Methods in molecular biology (Clifton, N.J.)
- Publish Date: 2006
- ISSN: 1064-3745
- Volume: 338
- Issue:
- Pages: 261-80
- Medium: Print
- Language: English
- Citation (JAMA): Ragoussis Jiannis, Field Simon, Udalova Irina A, et al. Quantitative Profiling of Protein-dna Binding on Microarrays.. Methods Mol. Biol. 2006;338:261-80
Abstract
Recent studies on genome-wide localization of transcription factor (TF) binding to DNA have shown that a large proportion of identified sequences do not contain consensus motifs predicted by databases of transcription factor binding sites, such as TRANSFAC. The main limitation of these databases is that they are based on a literature search of published examples of binding; consequently the data are not from a systematic survey and may be subject to sampling biases if investigators focused on particular motifs. Thus, there is an urgent need for systematic profiling of vertebrate transcription factor binding to DNA. We have developed a high-throughput platform for the quantitative analysis of protein-DNA interactions based on microarray technology.
Mesh Headings (Keywords): Base Sequence, Binding Sites, Cloning, Molecular, DNA, DNA-Binding Proteins, Humans, Octamer Transcription Factor-1, Oligonucleotide Array Sequence Analysis, Protein Binding, Recombinant Proteins, Transcription Factors
Check for Full Text / PubMed Unique Identifier (PMID): 16888364
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