Receptor-induced Activation of Drosophila Trp Gamma by Polyunsaturated Fatty Acids.
From: Institut für Pharmakologie, Charité Campus Benjamin Franklin, Thielallee 69-73, 14195 Berlin, Germany.
The Journal of biological chemistry
- Publish Date: Oct 2006
- ISSN: 0021-9258
- Volume: 281
- Issue: 40
- Pages: 29693-702
- Medium: Print
- Language: English
- Citation (JAMA): Jörs Simone, Kazanski Victor, Foik Anna, et al. Receptor-induced Activation of Drosophila Trp Gamma by Polyunsaturated Fatty Acids.. J. Biol. Chem. Oct 2006;281:29693-702
Abstract
Cellular calcium homeostasis is regulated by hormones and neurotransmitters, resulting in the activation of a variety of proteins, in particular, channel proteins of the plasma membrane and of intracellular compartments. Such channels are, for example, TRP channels of the TRPC protein family that are activated by various mediators from receptor-stimulated signaling cascades. In Drosophila, two TRPC channels, TRP and TRPL, are involved in phototransduction. In addition, a third Drosophila TRPC channel, TRPgamma, has been identified and described as an auxiliary subunit of TRPL. Beyond it, our data show that heterologously expressed TRPgamma formed a receptor-activated, outwardly rectifying cation channel independent from TRPL co-expression. Analysis of the activation mechanism revealed that TRPgamma is activated by various polyunsaturated fatty acids generated in a phospholipase C- and phospholipase A(2)-dependent manner. The most potent activator of TRPgamma, the stable analogue of arachidonic acid, 5,8,11,14-eicosatetraynoic acid, induced currents in single channel recordings. Here we show that upon heterologous expression TRPgamma forms a homomeric channel complex that is activated by polyunsaturated fatty acids as mediators of receptor-dependent signaling pathways. Reverse transcription PCR analysis showed that TRPgamma is expressed in Drosophila heads and bodies. Its body-wide expression pattern and its activation mechanism suggest that TRPgamma forms a fly cation channel responsible for the regulation of intracellular calcium in a variety of hormonal signaling cascades.
Mesh Headings (Keywords): Animals, Calcium Signaling, Cell Line, Drosophila Proteins, Drosophila melanogaster, Fatty Acids, Unsaturated, Humans, Insect Hormones, RNA, Messenger, Transient Receptor Potential Channels
Check for Full Text / PubMed Unique Identifier (PMID): 16901908
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