Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors.
From: Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.
Cell
- Publish Date: Aug 2006
- ISSN: 0092-8674
- Volume: 126
- Issue: 4
- Pages: 663-76
- Medium: Print
- Language: English
- Citation (JAMA): Takahashi Kazutoshi, Yamanaka Shinya, et al. Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors.. Cell Aug 2006;126:663-76
Abstract
Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was dispensable. These cells, which we designated iPS (induced pluripotent stem) cells, exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Subcutaneous transplantation of iPS cells into nude mice resulted in tumors containing a variety of tissues from all three germ layers. Following injection into blastocysts, iPS cells contributed to mouse embryonic development. These data demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors.
Mesh Headings (Keywords): Adult, Animals, Cell Differentiation, Cell Transplantation, Cells, Cultured, DNA-Binding Proteins, Embryo, Mammalian, Fibroblasts, Gene Expression Profiling, Homeodomain Proteins, Humans, Kruppel-Like Transcription Factors, Mice, Mice, Nude, Mice, Transgenic, Octamer Transcription Factor-3, Oligonucleotide Array Sequence Analysis, Pluripotent Stem Cells, Proto-Oncogene Proteins c-myc, Trans-Activators
Check for Full Text / PubMed Unique Identifier (PMID): 16904174
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