A Lentiviral Vector with Expression Controlled by E2f-1: a Potential Tool for the Study and Treatment of Proliferative Diseases.
From: Viral Vector Group, Laboratory of Genetics and Molecular Cardiology/LIM-13, University of São Paulo School of Medicine, Brazil. bstrauss@usp.br
Biochemical and biophysical research communications
- Publish Date: Oct 2006
- ISSN: 0006-291X
- Volume: 348
- Issue: 4
- Pages: 1411-8
- Medium: Print
- Language: English
- Citation (JAMA): Strauss Bryan E, Patrício Juliana Rotelli, de Carvalho Anna Carolina Vieira, et al. A Lentiviral Vector with Expression Controlled by E2f-1: a Potential Tool for the Study and Treatment of Proliferative Diseases.. Biochem. Biophys. Res. Commun. Oct 2006;348:1411-8
Abstract
We have constructed a lentiviral vector with expression limited to cells presenting active E2F-1 protein, a potential advantage for gene therapy of proliferative diseases. For the FE2FLW vector, the promoter region of the human E2F-1 gene was utilized to drive expression of luciferase cDNA, included as a reporter of viral expression. Primary, immortalized, and transformed cells were transduced with the FE2FLW vector and cell cycle alterations were induced with serum starvation/replacement, contact inhibition or drug treatment, revealing cell cycle-dependent changes in reporter activity. Forced E2F-1 expression, but not E2F-2 or E2F-3, increased reporter activity, indicating a major role for this factor in controlling expression from the FE2FLW virus. We show the utility of this vector as a reporter of E2F-1 and proliferation-dependent cellular alterations upon cytotoxic/cytostatic treatment, such as the introduction of tumor suppressor genes. We propose that the FE2FLW vector may be a starting point for the development of gene therapy strategies for proliferative diseases, such as cancer or restinosis.
Mesh Headings (Keywords): Animals, Cell Cycle, Cell Proliferation, Cells, Cultured, E2F Transcription Factors, E2F1 Transcription Factor, Gene Expression Regulation, Gene Therapy, Genes, Reporter, Genes, Tumor Suppressor, Genetic Vectors, Humans, Lentivirus, Promoter Regions (Genetics), Rats
Check for Full Text / PubMed Unique Identifier (PMID): 16920066
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