Neonatal and Adult Cd4+ Cd3- Cells Share Similar Gene Expression Profile, and Neonatal Cells Up-regulate Ox40 Ligand in Response to Tl1a (Tnfsf15).
From: Medical Research Council Centre for Immune Regulation, Institute for Biomedical Research, Birmingham Medical School, Birmingham, United Kingdom.
Journal of immunology (Baltimore, Md. : 1950)
- Publish Date: Sep 2006
- ISSN: 0022-1767
- Volume: 177
- Issue: 5
- Pages: 3074-81
- Medium: Print
- Language: English
- Citation (JAMA): Kim Mi-Yeon, Toellner Kai-Michael, White Andrea, et al. Neonatal and Adult Cd4+ Cd3- Cells Share Similar Gene Expression Profile, and Neonatal Cells Up-regulate Ox40 Ligand in Response to Tl1a (Tnfsf15).. J. Immunol. Sep 2006;177:3074-81
Abstract
We report here the quantitative expression of a set of immunity-related genes, including TNF family members, chemokine receptors, and transcription factors, in a CD4+ CD3- accessory cell. By correlating gene expression between cell-sorted populations of defined phenotype, we show that the genetic fingerprint of these CD4+ CD3- cells is distinct from dendritic cells, plasmacytoid dendritic cells, T cells, B cells, and NK cells. In contrast, it is highly similar to CD4+ CD3- cells isolated from embryonic and neonatal tissues, with the exception that only adult populations express OX40L and CD30L. We have previously reported that IL-7 signals regulate CD30L expression. In the present study, we show that both neonatal and adult CD4+ CD3- cells express the TNF family member, death receptor 3 (TNFRSF25), and that addition of TL1A (TNFSF15), the ligand for death receptor 3, up-regulates OX40L on neonatal CD4+ CD3- cells. Finally, we demonstrate that this differentiation occurs in vivo: neonatal CD4+ CD3- cells up-regulate both CD30L and OX40L after adoptive transfer into an adult recipient.
Mesh Headings (Keywords): Aging, Animals, Animals, Newborn, Antigens, CD, Antigens, CD3, CD30 Ligand, CD4-Positive T-Lymphocytes, Cells, Cultured, DNA Fingerprinting, Gene Expression Profiling, Gene Expression Regulation, Developmental, Membrane Glycoproteins, Mice, RNA, Messenger, Receptors, Retinoic Acid, Receptors, Thyroid Hormone, Receptors, Tumor Necrosis Factor, Receptors, Tumor Necrosis Factor, Member 25, Signal Transduction, Spleen, Tumor Necrosis Factor Ligand Superfamily Member 15, Tumor Necrosis Factor-alpha, Tumor Necrosis Factors, Up-Regulation
Check for Full Text / PubMed Unique Identifier (PMID): 16920944
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