Polo-like Kinase 1-mediated Phosphorylation of the Gtp-binding Protein Ran is Important for Bipolar Spindle Formation.
From: Laboratory of Cancer Prevention, NCI at Frederick, Frederick, MD, USA; Nanobiology Program, CCR, NCI at Frederick, Frederick, MD, USA. yfeng@mail.ncifcrf.gov
Biochemical and biophysical research communications
- Publish Date: Oct 2006
- ISSN: 0006-291X
- Volume: 349
- Issue: 1
- Pages: 144-52
- Medium: Print
- Language: English
- Citation (JAMA): Feng Yang, Yuan Jin Hui, Maloid Sharon C, et al. Polo-like Kinase 1-mediated Phosphorylation of the Gtp-binding Protein Ran is Important for Bipolar Spindle Formation.. Biochem. Biophys. Res. Commun. Oct 2006;349:144-52
Abstract
Polo-like kinase functions are essential for the establishment of a normal bipolar mitotic spindle, although precisely how Plk1 regulates the spindle is uncertain. In this study, we report that the small GTP/GDP-binding protein Ran is associated with Plk1. Plk1 is capable of phosphorylating co-immunoprecipitated Ran in vitro on serine-135 and Ran is phosphorylated in vivo at the same site during mitosis when Plk1 is normally activated. Cell cultures over-expressing a Ran S135D mutant have significantly higher numbers of abnormal mitotic cells than those over-expressing either wild-type or S135A Ran. The abnormalities in S135D mutant cells are similar to cells over-expressing Plk1. Our data suggests that Ran is a physiological substrate of Plk1 and that Plk1 regulates the spindle organization partially through its phosphorylation on Ran.
Mesh Headings (Keywords): Animals, Binding Sites, Cell Cycle Proteins, Cell Line, Cell Line, Tumor, Dogs, Guanosine Triphosphate, Humans, Mitosis, Mutation, Phosphorylation, Protein Binding, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins, Serine, ran GTP-Binding Protein
Check for Full Text / PubMed Unique Identifier (PMID): 16930555
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